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Image Search Results
Journal: The Analyst
Article Title: Antibody Purification Via Affinity Membrane Chromatography Method Utilizing Nucleotide Binding Site Targeting With A Small Molecule
doi: 10.1039/c6an02145j
Figure Lengend Snippet: Effect of BSA contamination on antibody recovery and purity of the m-NBST column. A) Chromatograms of Rituximab (0.5 mg/mL) premixed with increasing BSA content; elution times for BSA and Rituximab are indicated as 1-3 min and 8-10 min, respectively. B) ELISA results illustrating percent antibody in the flow through and elution fractions. Data represents the means (±SD) of triplicate experiments. C) SDS-PAGE analysis showed zero indication of BSA contamination in recovered antibody fractions.
Article Snippet: A
Techniques: Enzyme-linked Immunosorbent Assay, SDS Page
Journal: The Analyst
Article Title: Antibody Purification Via Affinity Membrane Chromatography Method Utilizing Nucleotide Binding Site Targeting With A Small Molecule
doi: 10.1039/c6an02145j
Figure Lengend Snippet: Effect of contaminants from various sources on antibody recovery and purity achieved by the m-NBST column. A) Chromatograms of Rituximab (0.5 mg/mL) contaminated with 2 mg/mL BSA, H929 Cell Supernatant, H929 Cell Lysate, Mouse Ascites and 3T3 Cell Lysate. B) ELISA results illustrating percent antibody in flow through and elution fractions. Data represents the means (±SD) of triplicate experiments. C) SDS-PAGE analysis showed absence of no protein contamination in recovered antibody fractions.
Article Snippet: A
Techniques: Enzyme-linked Immunosorbent Assay, SDS Page